So here’s a quick post on how the IEF Electrophoresis is done…
We’ve hydrated our gels right? And the gels are placed on ceramic tray…
Then, the gels are placed and coated with mineral oil again (Cover fluid). The electrode rack is placed over the paper, NOT the gel. The reason is to avoid direct contact to the gel.
The placement of electrodes as follows…
Control Panel to run the gel electrophoresis…
Watch the videos for more information…
Part 1 shows the setting of gel into the ceramic wells
Part 2 shows the setting of voltage and time for the electrophoresis run
Hope this tutorials could benefit everyone… Sorry about the soft audio because Ms Tan’s voice is very soft… so… next time have to ask her to speak louder… haha
4 comments:
Thank you Richard. =)
This is interesting. In addition, for fun, I think you would like biology educational cartoons on VADLO search engine.
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Thanks a lot people... I hope you guys really learn a lot from it...
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